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標題: 阿拉伯芥蛋白激酶AtYAK1參與受光調控的生長與發育
Functional characterization of a plant dual-specificity tyrosine phosphorylation-regulated kinase, AtYAK1, in light-regulated growth and development
作者: 黃文郁
Wen-Yu Huang
關鍵字: DYRK;AtYAK1;概日韻律;光形態發生;果莢發育;DYRK;AtYAK1;circadian clock;photomorphogenesis;silique;fertility
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光線為植物生長發育重要的環境因子。對植物而言,適切地感受到外界的光線能幫助適應環境的轉變。在本實驗室先前的研究中,發現LWD1和LWD2為植物生理時鐘重要的調節因子,負責調控概日韻律和開花時間。本論文則進一步研究與LWDs結合的蛋白激酶AtYAK1。AtYAK1為雙專一性酪氨酸磷酸化蛋白激酶家族 [Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family] 的一員,其酵素活性區域具高度跨物種的相似性,為重要的生長調節因子。在本論文中,發現AtYAK1會與LWDs結合而拮抗其功能,以控制概日韻律及開花時間。除此之外,進一步發現AtYAK1促進由光所調控之光形態發生(photomorphogenesis)及果莢生長,證明AtYAK1對於光調控之生理反應扮演重要角色。期望本篇研究能進一步衍生,作為農作物轉譯科學之基石。

Light controls vegetative and reproductive development of plants. For a plant, sensing the light input properly ensures coordination with the ever-changing environment. Previously, we found that LIGHT-REGULATED WD1 (LWD1) and LWD2 regulate the circadian clock and photoperiodic flowering. Here we identified Arabidopsis YET ANOTHER KINASE1 (AtYAK1), an evolutionarily conserved protein and a member of dual-specificity tyrosine phosphorylation-regulated kinases (DYRKs), as an interacting protein of LWDs. Our study revealed that AtYAK1 is an important regulator for various light responses, including the circadian clock, photomorphogenesis and reproductive development. AtYAK1 could antagonize the function of LWDs in regulating the circadian clock and photoperiodic flowering. By examining phenotypes of atyak1, we found that AtYAK1 regulated light-induced period-length shortening and photomorphogenic development. Moreover, AtYAK1 mediated plant fertility especially under inferior light conditions including low light and short-day length. This study discloses a new regulator connecting environmental light to plant growth.
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