Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/97748
標題: 柑橘類黃酮柚皮素的止痛與抗發炎作用
Anti-Nociceptive and Anti-Inflammatory Effects of Citrus Flavanone Naringenin
作者: 田易靈
Yi-Ling Tien
關鍵字: 抗發炎作用;止痛;發炎;柚皮素;疼痛;Anti-inflammatory;Anti-nociceptive;Inflammation;Naringenin;Pain
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Rofecoxib attenuates both primary and secondary inflammatory hyperalgesia: A randomized, double blinded, placebo controlled crossover trial in the UV-B pain model. Pain 2005;113:316-22. 7. Vasudevan M, Gunnam KK, Parle M. Antinociceptive and anti-inflammatory effects of Thespesia populnea bark extract. J Ethnopharmacol 2007;109:264-70. 8. Cragg GM, Newman DJ. Natural products: A continuing source of novel drug leads. Biochim Biophys Acta 2013;1830:3670-95. 9. Badal S, Turfus S, Rajnarayanan R, Wilson-Clarke C, Sandiford SL. Analysis of natural product regulation of opioid receptors in the treatment of human disease. Pharmacol Ther 2017 Oct 31. [Epub ahead of print] 10. Mir IA, Tiku AB. Chemopreventive and therapeutic potential of 'naringenin,' a flavanone present in citrus fruits. Nutr Cancer 2015;67:27–42. 11. Mulvihill EE, Allister EM, Sutherland BG, Telford DE, Sawyez CG, Edwards JY, et al. Naringenin prevents dyslipidemia, apolipoprotein B overproduction, and hyperinsulinemia in LDL receptor-null mice with diet-induced insulin resistance. Diabetes 2009;58:2198-210. 12. Assini JM, Mulvihill EE, Burke AC, Sutherland BG, Telford DE, Chhoker SS, et al. Naringenin prevents obesity, hepatic steatosis, and glucose intolerance in male mice independent of fibroblast growth factor 21. Endocrinology 2015;156:2087-102. 13. Ahmed OM, Hassan MA, Abdel-Twab SM, Abdel Azeem MN. Navel orange peel hydroethanolic extract, naringin and naringenin have anti-diabetic potentials in type 2 diabetic rats. Biomed Pharmacother 2017;94:197-205. 14. Lulu SS, Thabitha A, Vino S, Priya AM, Rout M. Naringenin and quercetin--potential anti-HCV agents for NS2 protease targets. Nat Prod Res 2016;30:464-8. 15. Park HJ, Choi YJ, Lee JH, Nam MJ. Naringenin causes ASK1-induced apoptosis via reactive oxygen species in human pancreatic cancer cells. Food Chem Toxicol 2017;99:1-8. 16. Shi Y, Dai J, Liu H, Li RR, Sun PL, Du Q, et al. Naringenin inhibits allergen-induced airway inflammation and airway responsiveness and inhibits NF-kappaB activity in a murine model of asthma. Physiol Pharmacol 2009;87:729-3. 17. Moon PD, Choi IH, Kim HM. Naringenin suppresses the production of thymic stromal lymphopoietin through the blockade of RIP2 and caspase-1 signal cascade in mast cells. Eur J Pharmacol 2011;671:128-32. 18. Chao CL, Weng CS, Chang NC, Lin JS, Kao ST, Ho FM. Naringenin more effectively inhibits inducible nitric oxide synthase and cyclooxygenase-2 expression in macrophages than in microglia. Nutr Res 2010;30:858-64. 19. Li YR, Chen DY, Chu CL, Li S, Chen YK, Wu CL, et al. Naringenin inhibits dendritic cell maturation and has therapeutic effects in a murine model of collagen-induced arthritis. J Nutr Biochem 2015;26:1467-78. 20. Wang HK, Yeh CH, Iwamoto T, Satsu H, Shimizu M, Totsuka M. Dietary flavonoid naringenin induces regulatory T cells via an aryl hydrocarbon receptor mediated pathway. J Agric Food Chem 2012;60:2171-8. 21. Zhu L, Wang J, Wei T, Gao J, He H, Chang X, et al. Effects of Naringenin on inflammation in complete freund's adjuvant-induced arthritis by regulating Bax/Bcl-2 balance. Inflammation 2015;38:245-51. 22. Wang CC, Guo L, Tian FD, An N, Luo L, Hao RH, et al. Naringenin regulates production of matrix metalloproteinases in the knee-joint and primary cultured articular chondrocytes and alleviates pain in rat osteoarthritis model. J Med Biol Res 2017;50:e5714. 23. Rajadurai M, Stanely Mainzen Prince P. Preventive effect of naringin on cardiac markers, electrocardiographic patterns and lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats. Toxicology. 2007;230:178-88. 24. Singh D, Chopra K. Effect of trimetazidine on renal ischemia/reperfusion injury in rats. Pharmacol Res. 2004;50:623-9. 25. 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摘要: 
柚皮素(Naringenin),一種被發現在柑橘類水果中的類黃酮,具有顯著多樣的藥理學性質。在本研究中,我們設計有關柚皮素對於止痛與抗發炎作用的相關研究。在大鼠與小鼠身上進行熱板測試、醋酸誘導扭動與甩尾測定來評估柚皮素的抗傷害作用。另外,透過角叉菜誘導的大鼠足腫脹測試來進行柚皮素抗發炎作用。我們發現口服柚皮素(100與200 mg/kg)的老鼠顯著延遲了對於熱板試驗所產生的熱刺激的反應時間,並且甩尾測定與醋酸誘導扭體次數也相對減少。另外,柚皮素在角叉菜誘導爪腫脹的測試中顯著抑制大鼠足腫脹,發揮抗發炎作用。我們的研究結果支持柚皮素具有止痛和抗發炎作用的治療潛力,並且可以進一步用於疼痛與發炎相關疾病的藥物開發。

Naringenin, a flavonoid found in citrus fruits, has notably diverse pharmacological properties. In the present study, we designed to investigate the anti-nociceptive and anti-inflammatory effects of the naringenin.
The anti-nociceptive effect was evaluated using hot-plate, acetic acid-induced writhing, and tail-flick assays in mice and rats. In addition, the anti-inflammatory effect was examined by carrageenan induced paw edema test in rats.
Naringenin (100, 200 mg/kg, oral administration) significantly delayed the reaction time of mice to the thermal stimulation generated with the hot-plate, and tail-flick unit and reduced acetic acid-induced writhing response in mice. In addition, naringenin significantly repressed the paw edema induced by carrageenan in rats, exerting its anti-inflammatory effect.
Our results support that the naringenin has therapeutic potential with anti-nociceptive and anti-inflammatory properties and can be further exploitable for drug development of pain and inflammatory related diseases.
URI: http://hdl.handle.net/11455/97748
Rights: 同意授權瀏覽/列印電子全文服務,2021-08-10起公開。
Appears in Collections:生命科學院碩士在職專班

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