Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/97809
標題: 在蘿蔔硫素抗胃癌效應中MCL-1上調之生物意義
Biological Significance of MCL-1 Upregulation in The Anti-Gastric Cancer Effect of Sulforaphane
作者: 李佳璇
Chia-Hsuan Li
關鍵字: 胃癌;蘿蔔硫素;MCL-1;ERK;NF-κB;抗藥性;Gastric cancer;Sulforaphane;MCL-1;ERK;NF-κB;Drug resistance
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摘要: 
胃癌為全球癌症死因排名第四的致命惡性腫瘤;在台灣,胃癌為癌症致死率中排名第七,因此需要更有效的治療。蘿蔔硫素是一種富含在十字花科植物 (如花椰菜和花椰菜嫩芽) 中的異硫氰酸酯 (isothiocyanate),文獻顯示蘿蔔硫素在不同類型的癌症中具有抗癌、抗發炎和抗氧化作用。本實驗室過去研究指出,蘿蔔硫素透過抑制存活路徑JAK2-STAT3-BCL-2達到抗胃癌效果。有趣的是,我們在胃癌細胞株AGS和MKN45中發現抗凋亡蛋白MCL-1的表現量隨著蘿蔔硫素濃度增加而呈現依賴性上升。先前研究已知MCL-1的上調節在許多癌症中與抗藥性有關。因此,本研究中,我們探討在處理蘿蔔硫素的胃癌中,MCL-1之分子機制與生物意義。我們發現:若將MCL-1 knockdown,可提升蘿蔔硫素對胃癌細胞的細胞毒性,另外,使用UMI-77 (MCL-1抑制劑) 亦可得到相同結果,並明顯增加蘿蔔硫素對胃癌細胞凋亡的誘導,顯示蘿蔔硫素上調節MCL-1表現量引起細胞對自身之抗藥性。此外,我們發現蘿蔔硫素會誘導ERK活化,因此使用U0126 抑制ERK活化,而MCL-1表現量也明顯受到抑制,顯示蘿蔔硫素可能透過ERK活化使MCL-1蛋白穩定性顯著增加。另外一方面,胃癌細胞處理蘿蔔硫素會下調節IκBα表現,並促進p-p65表現,顯示蘿蔔硫素可活化NF-κB活性,而NF-κB為MCL-1之轉錄活化因子。我們使用IκBα super repressor以防止IκBα降解,顯示蘿蔔硫素透過活化NF-κB促使下游MCL-1轉錄活性增加。綜合上述,發現在胃腺癌細胞中,蘿蔔硫素透過活化ERK及NF-κB訊號路徑上調節MCL-1以產生對蘿蔔硫素自身的抗藥性。我們的研究結果為日後蘿蔔硫素合併MCL-1抑制劑做為優化胃癌治療效果之新策略提供理論基礎。

Gastric cancer is the forth most lethal malignancy at present with leading cause of cancer-related death worldwide. In Taiwan, gastric cancer is the seventh leading cause of cancer-related death, thus highlighting the demand for more effective therapeutics. Sulforaphane is a cruciferous vegetables-derived isothiocyanate such as broccoli and broccoli sprouts, which exhibits effective anti-cancer, anti-inflammatory and anti-oxidative actions in various cancers. Our previous research has indicated that the anti-gastric cancer effect of sulforaphane by suppressing the JAK2-STAT3-BCL-2 survival pathway. Interestingly, we found that the level of antiapoptotic MCL-1 was clearly upregulated in sulforaphane-treated gastric cells (AGS and MKN45) in a dose-dependent manner. However, upregulation of MCL-1 has been shown to be associated with drug resistance in various cancers. In this study, we investigated the molecular mechanism and biological significance of MCL-1 in sulforaphane-treated gastric cancer. The cytotoxicity of sulforaphane on gastric cancer cells can be enhanced by knockdown of MCL-1 in gastric cancer cells. Furthermore, the same results were observed with UMI-77 (MCL-1 inhibitor) treatment significantly increase the induction of apoptosis in sulforaphane-treated gastric cancer, suggesting that sulforaphane upregulates MCL-1 to cause its own resistance. In addition, we found that sulforaphane treatment induced ERK activation. We used U0126 to inhibit ERK activation while the expression of MCL-1 is also significantly suppressed, suggesting that sulforaphane treatment led to a marked increase in MCL-1 protein stability, most likely through inducing ERK activation. Furthermore, the level of IκBα was downregulated in sulforaphane-treated gastric cells, indicating that sulforaphane could activate the activity of NF-κB, the transcription factor of MCL-1. We used IκBα super-repressor, which prevents IκBα from degradation, show that sulforaphane treatment promotes the transcriptional activity of MCL-1 by NF-κB activation. In conclusion, upregulation of MCL-1 through ERK and NF-κB pathway are the causes of resistance to sulforaphane in gastric cancer. Our results therefore to provide a new strategy for a better treatment of gastric cancer using sulforaphane in combination with MCL-1 inhibition as a theoretical reference.
URI: http://hdl.handle.net/11455/97809
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