Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/97815
標題: 以長效型微脂體包覆近紅外光螢光物diglucosamide-SIDAG診斷及治療乳癌
Encapsulation of Near-infrared Fluorophore, Diglucosamide-SIDAG, by Liposome as Breast Cancer Theranostics
作者: 歐書翰
Shu-Han Ou
關鍵字: 近紅外光螢光物;微脂體;奈米金粒子;光熱治療;光動力治療;diglucosamide-SIDAG;Liposome;photodynamic therapy;photothermal therapy
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摘要: 
此研究目的為利用長效型微脂體包覆光敏藥物diglucosamide-SIDAG和親水性金奈米粒子形成一奈米複合物,作為乳癌診斷治療劑之探討。利用長效型微脂體包覆diglucosamide-SIDAG使其維持高濃度狀態,受光激發後,經紅外線熱像儀和1,3-diphenylisobenzofuran(DPBF)單態氧檢測法確認可提升其光熱及光動力治療能力。相較之下,不同尺寸之親水性金奈米粒子產生光激發升溫變化,隨粒徑增加而上升。
細胞存活度實驗中,人類乳腺癌MDA-MB-231細胞經和包覆diglucosamide-SIDAG微脂體共培養並受光激發下,其細胞存活率大幅下降16%。為了確認其光啟動治療機制,利用單重態氧清除劑NaN3,可顯著提升接觸受光激發包覆diglucosamide-SIDAG微脂體的細胞存活率。比較微脂體包覆與否對螢光物傳輸進入細胞質內位置,經螢光顯微鏡觀察發現未包覆螢光物非選擇性散佈於細胞質內,受包覆螢光物則選擇性累積於粒腺體周圍。

The aim of this study was to use long-term circulation liposome coated photosensitive drug - diglucosamide-SIDAG and hydrophilic gold nanoparticles to form a nanocomposite as a diagnostic drug for breast cancer. Diglucosamide-SIDAG was coated with long-term circulation liposome to maintain its high concentration state. Upon activation by light, it was confirmed by infrared thermal imager and 1,3-diphenylisobenzofuran (DPBF) singlet oxygen detection method to enhance its photothermal and photodynamic treatment ability. In contrast, hydrophilic gold nanoparticles of different sizes produce photoexcitation changes in temperature, which increase with increasing particle size.
In the cell viability assay, human breast cancer MDA-MB-231 cells were co-cultured with diglucosamide-SIDAG in liposome and activated by light, and their cell viability decreased by 16%. In light initiated treatment mechanism, the single-state oxygen scavenger NaN3 was used to significantly increase the cell viability of the exposed photo-excited coated diglucosamide-SIDAG in liposome. Localization of SIDAG in cytosol, the fluorescent material is transported into the cytoplasm. It was observed by fluorescence microscopy that the uncoated fluorescent material was non-selectively dispersed in the cytoplasm, and the coated fluorescent material selectively accumulated around mitochondria.
URI: http://hdl.handle.net/11455/97815
Rights: 同意授權瀏覽/列印電子全文服務,2021-08-21起公開。
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