Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/97821
標題: 蘭嶼肉桂的 Isokotomolide A 和 Secokotomolide A 誘導黑色素瘤細胞自噬和凋亡並抑制體內和體外轉移
Isokotomolide A and Secokotomolide A from Cinnamomum kotoense induce autophagy and apoptosis and inhibit melanoma metastasis in vivo and in vitro
作者: 黃俊穎
Jyun-Ying Huang
關鍵字: 黑色素瘤;蘭嶼肉桂;凋亡;自噬;melanoma;Cinnamomum kotoense;apoptosis;autophagy
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摘要: 
黑色素瘤是一種具有高致死性的侵襲性癌症,為了尋找新的抗癌劑,從蘭嶼肉桂中分離出的isokotomolide A和secokotomolide A是對人類黑色素瘤的潛在生物活性劑。細胞增生試驗測定顯示經過isokotomolide A和secokotomolide A處理的正常人體皮膚細胞有高生存力。在B16F10,A2058,MeWo和A375黑色素瘤細胞中進一步驗證了兩者都具有強烈的抗黑素瘤作用。傷口癒合試驗呈現出其優異的抗黑色素瘤遷移效應。透過吖啶橙染色和西方點墨法和定量即時聚合酶鏈鎖反應,證實其誘導自噬的效果。我們透過使用annexin V-FITC / PI雙重染色,去驗證凋亡現象。同時也利用其誘導細胞週期停滯和造成DNA損傷。蛋白質表現證實caspase激活被誘導。並進一步做活體實驗,透過組織病理學分析染色去驗證有抑制腫瘤細胞生長的效果。在這項研究中,我們證實了isokotomolide A和secokotomolide A能通過早期的自噬作用和後期的凋亡作用來誘導黑色素瘤細胞死亡。

Melanoma is an aggressive cancer with high lethality, to find new anticancer agent, we identified isokotomolide A and secokotomolide A isolated from Cinnamomum kotoense to be potential bioactive agents against human melanoma but none anti-oxidant. Cell proliferation assay displayed isokotomolide A and secokotomolide A treated in the normal human skin cells showed high viabilities. It also verified both two of them possess strong anti-melanoma effect in dose-dependent manners, especially on B16F10, A2058, MeWo and A375 cells. Wound healing assay presented their excellent anti-migratory effects. Through AO staining and Western blot, we confirmed the autophagy induced by treatment. By using annexin V- FITC/PI double-stain, the apoptosis was confirmed. They can also induce cell cycle arrest and DNA damage. Western blot demonstrated caspase cascade activation were induced. To further evaluate in vivo experiments, through histopathological staining to verify the inhibition of tumor cell growth. Within this study, we confirmed isokotomolide A and secokotomolide A induce melanoma cells death via early autophagy and late apoptosis process.
URI: http://hdl.handle.net/11455/97821
Rights: 同意授權瀏覽/列印電子全文服務,2021-08-21起公開。
Appears in Collections:生醫工程研究所

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