Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/98120
標題: 水稻IR64黃胚乳突變體AZ1180之遺傳研究與黃金香米之育成
Genetic study on a yellow endosperm mutant, AZ1180 of IR64 rice variety, and breeding to pyramid the aroma trait
作者: 游鎮州
Cheng-Chou Yu
關鍵字: 水稻;黃金香米;分子標誌輔助育種;疊氮化鈉;Rice;golden aroma rice;marker-assisted selection (MAS);sodium azide
引用: 林岡諭。2013。秈稻IR64 之香氣突變體IR64A 之遺傳分析與基因定位。碩士論文。台中:國立中興大學農藝學研究所。 田妮可。2014。水稻台農67號糯性突變體與轉位子Pong轉位關係之探討。碩士論文。台中:國立中興大學農藝學研究所。 吳永培、廖大經。2017。台灣有色米之發展現況及因應措施。技術服務 28:1-5。 林宏諭。2012。論文題目 1:利用代謝體學策略研究黃金米色素 論文題目 2: 利用質譜學進行米糠萃取精油之活性成分分析。碩士論文。台中:國立中興大學分子生物學研究所。 施怡如、鄭統隆、曾東海、王強生。2004。有色水稻中花青素的種類及其功能探討。中華農業研究 53:221-228。 楊仕劤。2009。台農 76 號 (黃米) 色素成分探討與 ALDH 突變基因之表現。碩士論文。台中:東海大學食品科學系 賴喜美。2014。米食多元化加工技術-米穀粉之研究與開發。農業生技產業季刊:56-62。 沈一、刘玲珑、刘世家、程治军、江玲、万建民。2011。水稻 OsALDH7 基因的功能初步分析。中国科技论文在线。 沈一、刘玲珑、刘世家、程治军、江玲、万建民。2011。水稻黄胚乳突变体 JMW 的基因定位与克隆。中国科技论文在线。 Adamu, A. and H. Aliyu. 2007. Morphogical effects of sodium azide on tomato (Lycopersicon esculentum Mill). Science World Journal 2. Allard, R.W. 1999. Principles of plant breedingJohn Wiley & Sons. Arenaz, P., L. Hallberg, F. Mancillas, G. Gutierrez and S. Garcia. 1989. Sodium azide mutagenesis in mammals: inability of mammalian cells to convert azide to a mutagenic intermediate. Mutation Research Letters 227: 63-67. Ashikari, M., J. Wu, M. Yano, T. Sasaki and A. Yoshimura. 1999. Rice gibberellin-insensitive dwarf mutant gene Dwarf 1 encodes the α-subunit of GTP-binding protein. Proceedings of the National Academy of Sciences 96: 10284-10289. Berner, D.K. and B. Hoff. 1986. Inheritance of scent in American long grain rice. Crop Science 26: 876-878. Bhattacharjee, P., R.S. Singhal and P.R. Kulkarni. 2002. Basmati rice: a review. International journal of food science & technology 37: 1-12. Bradbury, L.M., T.L. Fitzgerald, R.J. Henry, Q. Jin and D.L. Waters. 2005. The gene for fragrance in rice. Plant Biotechnology Journal 3: 363-370. Buttery, R.G., L.C. Ling, B.O. Juliano and J.G. Turnbaugh. 1983. Cooked rice aroma and 2-acetyl-1-pyrroline. Journal of Agricultural and Food Chemistry 31: 823-826. Che, R., H. Tong, B. Shi, Y. Liu, S. Fang, D. Liu, Y. Xiao, B. Hu, L. Liu and H. Wang. 2016. Control of grain size and rice yield by GL2-mediated brassinosteroid responses. Nature plants 2: 15195. Chen, S., J. Wu, Y. Yang, W. Shi and M. Xu. 2006. The fgr gene responsible for rice fragrance was restricted within 69kb. Plant Science 171: 505-514. Collard, B.C. and D.J. Mackill. 2008. Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Philosophical Transactions of the Royal Society B: Biological Sciences 363: 557-572. Committee, I.-I.R.A. and I.B.f.P.G. Resources. 1980. Descriptors for Rice, Oryza Sativa LInt. Rice Res. Inst. Cox, T., W. Raupp and B. Gill. 1994. Leaf rust-resistance genes Lr41, Lr42, and Lr43 transferred from Triticum tauschii to common wheat. Crop science 34: 339-343. DE VRIES, M.E., P.A. Leffelaar, N. Sakane, B.V. Bado and K.E. Giller. 2011. Adaptability of irrigated rice to temperature change in Sahelian environments. Experimental Agriculture 47: 69-87. Dellaporta, S.L., J. Wood and J.B. Hicks. 1983. A plant DNA minipreparation: version II. Plant molecular biology reporter 1: 19-21. Fan, C., Y. Xing, H. Mao, T. Lu, B. Han, C. Xu, X. Li and Q. Zhang. 2006. GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theoretical and Applied Genetics 112: 1164-1171. Fehr, W.R. 1987. Principles of cultivar development. Volume 1. Theory and techniqueMacmillan publishing company. Fitzgerald, T.L., D.L. Waters and R.J. Henry. 2009. Betaine aldehyde dehydrogenase in plants. Plant Biology 11: 119-130. Gahan, L.J., Y.-T. Ma, M.L. MacGregor Coble, F. Gould, W.J. Moar and D.G. Heckel. 2005. Genetic basis of resistance to Cry1Ac and Cry2Aa in Heliothis virescens (Lepidoptera: Noctuidae). Journal of Economic Entomology 98: 1357-1368. Gao, C. and B. Han. 2009. Evolutionary and expression study of the aldehyde dehydrogenase (ALDH) gene superfamily in rice (Oryza sativa). Gene 431: 86-94. Goto, T. and T. Kondo. 1991. Structure and molecular stacking of anthocyanins—flower color variation. Angewandte Chemie International Edition 30: 17-33. Grant, W.F. and M.F. Salamone. 1994. Comparative mutagenicity of chemicals selected for test in the International Program on Chemical Safety's collaborative study on plant systems for the detection of environmental mutagens. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 310: 187-209. Gu, B., T. Zhou, J. Luo, H. Liu, Y. Wang, Y. Shangguan, J. Zhu, Y. Li, T. Sang, Z. Wang and B. Han. 2015. An-2 Encodes a Cytokinin Synthesis Enzyme that Regulates Awn Length and Grain Production in Rice. Molecular Plant 8: 1635-1650. doi:https://doi.org/10.1016/j.molp.2015.08.001. Hittalmani, S., A. Parco, T. Mew, R. Zeigler and N. Huang. 2000. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theoretical and Applied Genetics 100: 1121-1128. Hong, Z., M. Ueguchi-Tanaka, K. Umemura, S. Uozu, S. Fujioka, S. Takatsuto, S. Yoshida, M. Ashikari, H. Kitano and M. Matsuoka. 2003. A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. The Plant Cell 15: 2900-2910. Hu, G., D. Zhang, H. Pan, B. Li, J. Wu, X. Zhou, Q. Zhang, L. Zhou, G. Yao and J. Li. 2011. Fine mapping of the awn gene on chromosome 4 in rice by association and linkage analyses. Chinese science bulletin 56: 835-839. Hu, J., Y. Wang, Y. Fang, L. Zeng, J. Xu, H. Yu, Z. Shi, J. Pan, D. Zhang and S. Kang. 2015. A rare allele of GS2 enhances grain size and grain yield in rice. Molecular plant 8: 1455-1465. Huang, N., E. Angeles, J. Domingo, G. Magpantay, S. Singh, G. Zhang, N. Kumaravadivel, J. Bennett and G. Khush. 1997. Pyramiding of bacterial blight resistance genes in rice: marker-assisted selection using RFLP and PCR. Theoretical and Applied Genetics 95: 313-320. Huang, T.-C., C.-S. Teng, J.-L. Chang, H.-S. Chuang, C.-T. Ho and M.-L. Wu. 2008. Biosynthetic mechanism of 2-acetyl-1-pyrroline and its relationship with Δ1-pyrroline-5-carboxylic acid and methylglyoxal in aromatic rice (Oryza sativa L.) callus. Journal of agricultural and food chemistry 56: 7399-7404. Huang, W., X. Ma, Q. Wang, Y. Gao, Y. Xue, X. Niu, G. Yu and Y. Liu. 2008. Significant improvement of stress tolerance in tobacco plants by overexpressing a stress-responsive aldehyde dehydrogenase gene from maize (Zea mays). Plant molecular biology 68: 451-463. Ishimaru, K., N. Hirotsu, Y. Madoka, N. Murakami, N. Hara, H. Onodera, T. Kashiwagi, K. Ujiie, B.-i. Shimizu and A. Onishi. 2013. Loss of function of the IAA-glucose hydrolase gene TGW6 enhances rice grain weight and increases yield. Nature genetics 45: 707. Itoh, H., T. Tatsumi, T. Sakamoto, K. Otomo, T. Toyomasu, H. Kitano, M. Ashikari, S. Ichihara and M. Matsuoka. 2004. A rice semi-dwarf gene, Tan-Ginbozu (D35), encodes the gibberellin biosynthesis enzyme, ent-kaurene oxidase. Plant molecular biology 54: 533-547. Jackson, R., J. Bradley Jr and J. Van Duyn. 2003. Field performance of transgenic cottons expressing one or two Bacillus thuringiensis endotoxins against bollworm, Helicoverpa zea (Boddie). Journal of Cotton Science. Jeng, T., T. Tseng, C. Wang, C. Chen and J. Sung. 2003. Starch biosynthesizing enzymes in developing grains of rice cultivar Tainung 67 and its sodium azide-induced rice mutant. Field crops research 84: 261-269. Joshi, R.K. and S. Nayak. 2010. Gene pyramiding-A broad spectrum technique for developing durable stress resistance in crops. Biotechnology and Molecular Biology Reviews 5: 51-60. Julia, C. and M. Dingkuhn. 2013. Predicting temperature induced sterility of rice spikelets requires simulation of crop-generated microclimate. European Journal of Agronomy 49: 50-60. Kaji, R., M. Okamoto, M. Sakai, R. Mizobuchi, K. Tamura, H. Hirabayashi, Y. Tamura, S. Fukaura, T. Tomimatsu and H. Nakano. 2010. 'Hatsuyamabuki', a new rice variety with yellow endosperm derived from gamma ray mutation. Breeding Research (Japan). Katiyar, S., S. Verulkar, G. Chandel, Y. Zhang, B. Huang and J. Bennett. 2001. Genetic analysis and pyramiding of two gall midge resistance genes (Gm-2 and Gm-6t) in rice (Oryza sativa L.). Euphytica 122: 327-334. Khan, S., F. Al-Qurainy and F. Anwar. 2009. Sodium azide: a chemical mutagen for enhancement of agronomic traits of crop plants. Environ. We Int. J. Sci. Tech 4: 1-21. Kharkwal, M.C., R.N. Pandey and S.E. Pawar. 2004. Mutation Breeding for Crop Improvement. In: H. K. Jain and M. C. Kharkwal, editors, Plant Breeding: Mendelian to Molecular Approaches. Springer Netherlands, Dordrecht. p. 601-645. Kihlman, B. 1959. The effect of respiratory inhibitors and chelating agents on the frequencies of chromosomal aberrations produced by X-rays in Vicia. The Journal of Cell Biology 5: 479-490. Kim, J.K., S.Y. Lee, S.M. Chu, S.H. Lim, S.-C. Suh, Y.-T. Lee, H.S. Cho and S.-H. Ha. 2010. Variation and correlation analysis of flavonoids and carotenoids in Korean pigmented rice (Oryza sativa L.) cultivars. Journal of agricultural and food chemistry 58: 12804-12809. Kirch, H.-H., D. Bartels, Y. Wei, P.S. Schnable and A.J. Wood. 2004. The ALDH gene superfamily of Arabidopsis. Trends in plant science 9: 371-377. Kirch, H.-H., S. Schlingensiepen, S. Kotchoni, R. Sunkar and D. Bartels. 2005. Detailed expression analysis of selected genes of the aldehyde dehydrogenase (ALDH) gene superfamily in Arabidopsis thaliana. Plant molecular biology 57: 315-332. Klášterská, I., A. Natarajan and C. Ramel. 1976. An interpretation of the origin of subchromatid aberrations and chromosome stickiness as a category of chromatid aberrations. Hereditas 83: 153-162. Kotchoni, S.O., J.C. Jimenez-Lopez, D. Gao, V. Edwards, E.W. Gachomo, V.M. Margam and M.J. Seufferheld. 2010. Modeling-dependent protein characterization of the rice aldehyde dehydrogenase (ALDH) superfamily reveals distinct functional and structural features. PloS one 5: e11516. Kotchoni, S.O., C. Kuhns, A. Ditzer, H.H. KIRCH and D. Bartels. 2006. Over‐expression of different aldehyde dehydrogenase genes in Arabidopsis thaliana confers tolerance to abiotic stress and protects plants against lipid peroxidation and oxidative stress. Plant, cell & environment 29: 1033-1048. Kovach, M.J., M.N. Calingacion, M.A. Fitzgerald and S.R. McCouch. 2009. The origin and evolution of fragrance in rice (Oryza sativa L.). Proceedings of the National Academy of Sciences 106: 14444-14449. Kumaravadivel, N., M. Uma, P. Saravanan and H. Suresh. 2006. Molecular marker-assisted selection and pyramiding genes for gall midge resistance in rice suitable for Tamil Nadu Region. ABSTRACTS-2nd International Rice Congress: 257. Kuo, S.-M., S.-Y. Chou, A.Z. Wang, T.-H. Tseng, F.-S. Chueh, H.E. Yen and C.-S. Wang. 2005. The betaine aldehyde dehydrogenase (BAD2) gene is not responsible for aroma trait of AS0420 rice mutant derived by sodium azide mutagenesis. Proceedings of the 5th international rice genetics symposium, IRRI, Philippines 166. Lande, R. and R. Thompson. 1990. Efficiency of marker-assisted selection in the improvement of quantitative traits. Genetics 124: 743-756. Liu, J., D. Liu, W. Tao, W. Li, S. Wang, P. Chen, S. Cheng and D. Gao. 2000. Molecular marker‐facilitated pyramiding of different genes for powdery mildew resistance in wheat. Plant Breeding 119: 21-24. Luo, J., H. Liu, T. Zhou, B. Gu, X. Huang, Y. Shangguan, J. Zhu, Y. Li, Y. Zhao and Y. Wang. 2013. An-1 encodes a basic helix-loop-helix protein that regulates awn development, grain size, and grain number in rice. The Plant Cell 25: 3360-3376. Mackill, D.J. and G.S. Khush. 2018. IR64: a high-quality and high-yielding mega variety. Rice 11: 18. doi:10.1186/s12284-018-0208-3. McCouch12, S., R. Nelson, J. Tohme and R. Zeigler. 1994. Mapping of blast resistance genes in rice. Rice blast disease: 167. Mew, T. 1987. Current status and future prospects of research on bacterial blight of rice. Annual review of phytopathology 25: 359-382. Moreau, L., A. Charcosset and A. Gallais. 1998. Marker-assisted selection efficiency in populations of finite size. Genetics 148: 1353-1365. Moreau, L., F. Lacoudre, A. Charcosset and A. Gallais. 1997. More on the efficiency of marker-assisted selection. Theoretical and applied genetics 95: 1181-1189. Nakano, H., S. Kosemura, T. Suzuki, K. Hirose, R. Kaji and M. Sakai. 2009. Oryzamutaic acid A, a novel yellow pigment from an Oryza sativa mutant with yellow endosperm. Tetrahedron Letters 50: 2003-2005. Nakano, H., S. Kosemura, M. Yoshida, R. Iwaura, T. Suzuki, R. Kaji and M. Sakai. 2010. Oryzamutaic acids H–J, new alkaloids from an Oryza sativa mutant with yellow endosperm. Tetrahedron Letters 51: 4953-4956. Nakano, H., S. Kosemura, M. Yoshida, T. Suzuki, R. Iwaura, R. Kaji, M. Sakai and K. Hirose. 2010. Oryzamutaic acids B–G, new alkaloids from an Oryza sativa mutant with yellow endosperm. Tetrahedron Letters 51: 49-53. Nakano, H., K. Tamura, M. Sakai, N. Ooka, M. Arakawa, K. Yagasaki, T. Minoda, S. Noda and M. Arai. 2011. Isolation and identification of oryzamutaic acids A and D from an Oryza sativa spontaneous mutant with yellow endosperm. Breeding science 61: 93-95. Narayanan, N., N. Baisakh, V. Cruz, S. Gnanamanickam, K. Datta and S. Datta. 2002. Molecular breeding for the development of blast and bacterial blight resistance in rice cv. IR50. Crop Science 42: 2072-2079. Nilan, R. 1975. Lack of chromosome breakage by azide in embryonic shoots and microspores of barley. BGN 5: 33. Owais, W. and A. Kleinhofs. 1988. Metabolic activation of the mutagen azide in biological systems. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 197: 313-323. Pinson, S. 1994. Inheritance of aroma in six rice cultivars. Crop Science 34: 1151-1157. Qi, P., Y.-S. Lin, X.-J. Song, J.-B. Shen, W. Huang, J.-X. Shan, M.-Z. Zhu, L. Jiang, J.-P. Gao and H.-X. Lin. 2012. The novel quantitative trait locus GL3. 1 controls rice grain size and yield by regulating Cyclin-T1; 3. Cell research 22: 1666. Raicu, P. and F. Mixich. 1992. Cytogenetic effects of sodium azide encapsulated in liposomes on heteroploid cell cultures. Mutation Research Letters 283: 215-219. Ribaut, J.-M., C. Jiang and D. Hoisington. 2002. Simulation experiments on efficiencies of gene introgression by backcrossing. Crop Science 42: 557-565. Rines, H. 1985. Sodium azide mutagenesis in diploid and hexaploid oats and comparison with ethyl methanesulfonate treatments. Environmental and experimental botany 25: 7-16. Rodrigues, S.M., M.O. Andrade, A.P.S. Gomes, F.M. DaMatta, M.C. Baracat-Pereira and E.P. Fontes. 2006. Arabidopsis and tobacco plants ectopically expressing the soybean antiquitin-like ALDH7 gene display enhanced tolerance to drought, salinity, and oxidative stress. Journal of experimental botany 57: 1909-1918. Sanchez, A., D. Brar, N. Huang, Z. Li and G. Khush. 2000. Sequence Tagged Site Marker-Assisted Selection for Three Bacterial Blight Resistance Genes in Rice Current address of N. Huang is: Applied Phytologics Inc., 4110 N. Freeway Blvd., Sacramento, CA 95834. Crop Science 40: 792-797. Sander, C., R. Nilan, A. Kleinhofs and B. Vig. 1978. Mutagenic and chromosome-breaking effects of azide in barley and human leukocytes. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 50: 67-75. Sasaki, A., M. Ashikari, M. Ueguchi-Tanaka, H. Itoh, A. Nishimura, D. Swapan, K. Ishiyama, T. Saito, M. Kobayashi and G.S. Khush. 2002. Green revolution: a mutant gibberellin-synthesis gene in rice. Nature 416: 701. Sasaki, A., H. Itoh, K. Gomi, M. Ueguchi-Tanaka, K. Ishiyama, M. Kobayashi, D.-H. Jeong, G. An, H. Kitano and M. Ashikari. 2003. Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant. Science 299: 1896-1898. Schneider, A., S. Walker, M. Sagan, G. Duc, T. Ellis and J. Downie. 2002. Mapping of the nodulation loci sym9 and sym10 of pea (Pisum sativum L.). Theoretical and Applied Genetics 104: 1312-1316. Servin, B., O.C. Martin and M. Mézard. 2004. Toward a theory of marker-assisted gene pyramiding. Genetics 168: 513-523. Shao, G., A. Tang, S. Tang, J. Luo, G. Jiao, J. Wu and P. Hu. 2011. A new deletion mutation of fragrant gene and the development of three molecular markers for fragrance in rice. Plant breeding 130: 172-176. Shen, Y., L. Liu, S. Liu, L. Jiang, C. Yang, Y. Lan and J. Wan. 2007. Identification and mapping of a golden endosperm gene in rice. Rice Genetics Newsletter 24: 25-27. Shen, Y., Y. Zhang, C. Yang, Y. Lan, L. Liu, S. Liu, Z. Chen, G. Ren and J. Wan. 2012. Mutation of OsALDH7 causes a yellow-colored endosperm associated with accumulation of oryzamutaic acid A in rice. Planta 235: 433-441. Shi, W., Y. Yang, S. Chen and M. Xu. 2008. Discovery of a new fragrance allele and the development of functional markers for the breeding of fragrant rice varieties. Molecular Breeding 22: 185-192. Shin, J.-H., S.-R. Kim and G. An. 2009. Rice aldehyde dehydrogenase7 is needed for seed maturation and viability. Plant physiology 149: 905-915. Si, L., J. Chen, X. Huang, H. Gong, J. Luo, Q. Hou, T. Zhou, T. Lu, J. Zhu and Y. Shangguan. 2015. OsSPL13 controls grain size in cultivated rice. Nature genetics 47: 447. Siddiq, E., L. Vemireddy and J. Nagaraju. 2012. Basmati rices: Genetics, breeding and trade. Agricultural Research 1: 25-36. Singh, R., G. Khush, U. Singh, A. Singh and S. Singh. 2000. Breeding aromatic rice for high yield, improved aroma and grain quality. P 71-106. Aromatic rices. Singh RK, Singh US, and Khush GS ed. Oxford & IBH Publishing Co. Pvt. Ltd. New Delhi. 289p. Singh, S., J. Sidhu, N. Huang, Y. Vikal, Z. Li, D. Brar, H. Dhaliwal and G. Khush. 2001. Pyramiding three bacterial blight resistance genes (xa5, xa13 and Xa21) using marker-assisted selection into indica rice cultivar PR106. Theoretical and Applied Genetics 102: 1011-1015. Sood, B. and E. Siddiq. 1978. A rapid technique for scent determination in rice. Indian Journal of Genetics and Plant Breeding (The) 38: 268-275. Sophos, N.A., A. Pappa, T.L. Ziegler and V. Vasiliou. 2001. Aldehyde dehydrogenase gene superfamily: the 2000 update. Chemico-biological interactions 130: 323-337. Sophos, N.A. and V. Vasiliou. 2003. Aldehyde dehydrogenase gene superfamily: the 2002 update. Chemico-biological interactions 143: 5-22. Suwannaporn, P. and A. Linnemann. 2008. RICE‐EATING QUALITY AMONG CONSUMERS IN DIFFERENT RICE GRAIN PREFERENCE COUNTRIES. Journal of Sensory Studies 23: 1-13. Tanabata, T., T. Shibaya, K. Hori, K. Ebana and M. Yano. 2012. SmartGrain: high-throughput phenotyping software for measuring seed shape through image analysis. Plant physiology: pp. 112.205120. Tanabe, S., M. Ashikari, S. Fujioka, S. Takatsuto, S. Yoshida, M. Yano, A. Yoshimura, H. Kitano, M. Matsuoka and Y. Fujisawa. 2005. A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length. The Plant Cell 17: 776-790. Vasiliou, V., A. Bairoch, K.F. Tipton and D.W. Nebert. 1999. Eukaryotic aldehyde dehydrogenase (ALDH) genes: human polymorphisms, and recommended nomenclature based on divergent evolution and chromosomal mapping. Pharmacogenetics and Genomics 9: 421-434. Wang, C.-S., T.-H. Tseng and C.-Y. Lin. 2002. Rice biotech research at the Taiwan Agricultural Research Institute. Asia-Pacific Biotech News 6: 950-956. Wang, Y., G. Xiong, J. Hu, L. Jiang, H. Yu, J. Xu, Y. Fang, L. Zeng, E. Xu and J. Xu. 2015. Copy number variation at the GL7 locus contributes to grain size diversity in rice. Nature genetics 47: 944. Wei, X., J. Xu, H. Guo, L. Jiang, S. Chen, C. Yu, Z. Zhou, P. Hu, H. Zhai and J. Wan. 2010. DTH8 suppresses flowering in rice, influencing plant height and yield potential simultaneously. Plant physiology 153: 1747-1758. Wen, J. and H. Liang. 1995. Effects of KCN and NaN_ (3) pretreatment on the cyanide-resistant respiration in tobacco callus. Acta Botanica Sinica 37: 711-717. Werner, K., W. Friedt and F. Ordon. 2005. Strategies for pyramiding resistance genes against the barley yellow mosaic virus complex (BaMMV, BaYMV, BaYMV-2). Molecular Breeding 16: 45-55. Yamamuro, C., Y. Ihara, X. Wu, T. Noguchi, S. Fujioka, S. Takatsuto, M. Ashikari, H. Kitano and M. Matsuoka. 2000. Loss of function of a rice brassinosteroid insensitive1 homolog prevents internode elongation and bending of the lamina joint. The Plant Cell 12: 1591-1605. Yan, W.-H., P. Wang, H.-X. Chen, H.-J. Zhou, Q.-P. Li, C.-R. Wang, Z.-H. Ding, Y.-S. Zhang, S.-B. Yu, Y.-Z. Xing and Q.-F. Zhang. 2011. A Major QTL, Ghd8, Plays Pleiotropic Roles in Regulating Grain Productivity, Plant Height, and Heading Date in Rice. Molecular Plant 4: 319-330. doi:https://doi.org/10.1093/mp/ssq070. Yang, S.-h., X.-l. Niu, D. Luo, C.-d. Chen, X. Yu, W. Tang, B.-r. Lu and Y.-s. Liu. 2012. Functional Characterization of an Aldehyde Dehydrogenase Homologue in Rice. Journal of Integrative Agriculture 11: 1434-1444. doi:http://dx.doi.org/10.1016/S2095-3119(12)60143-9. Ying, J.-Z., M. Ma, C. Bai, X.-H. Huang, J.-L. Liu, Y.-Y. Fan and X.-J. Song. 2018. TGW3, a Major QTL that Negatively Modulates Grain Length and Weight in Rice. Molecular plant 11: 750-753. Yoshihashi, T., N.T.T. Huong and H. Inatomi. 2002. Precursors of 2-acetyl-1-pyrroline, a potent flavor compound of an aromatic rice variety. Journal of agricultural and food chemistry 50: 2001-2004. Zhang, X., J. Wang, J. Huang, H. Lan, C. Wang, C. Yin, Y. Wu, H. Tang, Q. Qian and J. Li. 2012. Rare allele of OsPPKL1 associated with grain length causes extra-large grain and a significant yield increase in rice. Proceedings of the National Academy of Sciences 109: 21534-21539.
摘要: 
水稻 (Oryza sativa L.) 是世界的重要糧食作物之一,香米則在許多東南亞國家受到當地人民的青睞,因其在烹煮時會產生特殊的香氣,所以其價格在國際市場也較一般白米來得高。AZ0001為本實驗室利用疊氮化鈉誘變秈稻 IR64 品種於M5¬¬世代發現之香氣突變體。AZ1180則是於2014年二期作發現自IR64 誘變庫發現之黃胚乳突變體。經研究發現,在AZ0001的第八條染色體的長臂上的badh2為其主要控制香味的基因;但是目前於AZ1180胚乳內所含的黃色化合物須更進一步進行鑑定與分析才能確定。本試驗於2014年二期作將AZ1180與AZ0001進行正反交。其F1穀粒皆都呈現白色,因此推測該黃色胚乳為隱性性狀。於F2時調查其胚乳性狀,並進行卡方檢定,發現正反交結果都一致,符合63白 : 1 黃的遺傳分離比,因此推測控制黃色胚乳的基因有三個,而且該性狀屬於核基因的控制。本論文使用分子標誌輔助育種 (marker-assisted selection, MAS) 與傳統譜系育種法,以育成具有優良性狀之黃金香米新品系。於2017年2期作挑出10個黃金香米優良品系,並於2018年1期作進行試量產,所育成之黃金香米品系已達到第7世代,它們分別都具有又黃又香、性狀良好與不同之粒型。本試驗所育成之新品系黃金香米預計將於未來問世。

Rice (Oryza sativa L.) is one of the major food crops in the world. AZ0001 (IR64A) is an aroma mutant and AZ1180 is a yellow endosperm mutant both screened from the IR64 mutant pool developed by using sodium azide mutagenesis. AZ0001 contains a single recessive allele, badh2.1, however, the inheritance and compound of the yellow endosperm of AZ1180 are still unknown. The cross AZ0001/AZ1180 and its reciprocal cross were conducted to study the inheritance of yellow endosperm in AZ1180 and to combine the aroma trait to develop a new variety with both traits. In F1 population of both crosses, all the endosperm showed white indicated that the yellow endosperm is recessive inheritance. The endosperm color of two F2 populations, AZ1180/AZ0001 and AZ0001/AZ1180 both segregated as 63 white: 1 yellow (χ2=0.5169 and 0.4453) suggest that the yellow endosperm is controlled by three recessive genes in the nucleus. Through the marker-assisted selection (MAS) breeding and traditional pedigree breeding. We have proceeded to the F7 generation and obtained 10 pure lines with yellow endosperm, aroma, and good agronomic traits with various grain shapes. New rice variety of golden aroma is expected to be released in the future.
URI: http://hdl.handle.net/11455/98120
Rights: 同意授權瀏覽/列印電子全文服務,2021-08-30起公開。
Appears in Collections:國際農學碩士學位學程

Files in This Item:
File SizeFormat Existing users please Login
nchu-107-7104030504-1.pdf27.2 MBAdobe PDFThis file is only available in the university internal network    Request a copy
Show full item record
 
TAIR Related Article

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.