Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/98168
DC FieldValueLanguage
dc.contributor黃振文zh_TW
dc.contributorJenn-Wen Huangen_US
dc.contributor.author高如沄zh_TW
dc.contributor.authorJu-Yun Kaoen_US
dc.contributor.other植物醫學暨安全農業碩士學位學程zh_TW
dc.date2019zh_TW
dc.date.accessioned2019-03-22T06:27:55Z-
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Induction of phenylalanine deaminase by light and its relation to chlorogenic acid synthesis in potato tuber tissue. Plant Physiol. 40: 779-784.zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/98168-
dc.description.abstract在高溫潮濕的環境下,有機農場栽培的小白菜 (Brassica rapa subsp. chinensis)常遭受白菜炭疽病菌 (Colletotrichum higginsianum Sacc.) 為害,引起植株葉片出現灰褐色圓形的壞疽斑,致使受害植株喪失商品價值。近年來,社會大眾的環保意識抬頭,重視農產品的品質安全已成為國人追求的目標,因此本研究主要目的在於探討植物激活蛋白與乳化葵花油防治小白菜炭疽病的功效,祈有助於減少化學農藥的用量。本研究室林氏等人 (2017) 曾證明鏈格孢菌Alternaria alternata (Fr.) Keissl. (ALA) 的菌絲蛋白萃取液可顯著降低胡瓜炭疽病與白菜立枯病等病害的發生,並以基因轉殖之Escherichia coli ALA-01 clone加入IPTG (Isopropyl β-D-1-thiogalactopyranoside),成功生產Ape 1蛋白激活子。由於IPTG價格昂貴,本研究嘗試以1 mM半乳糖替代IPTG作為生產蛋白激活子的誘導物,結果可顯著提高Ape 1蛋白產量約20.5 %,且在製備培養基時即可加入半乳糖作為自發性誘導的材料,無需於生產過程監控菌量的變化。利用Bradford蛋白質定量法將Ape 1蛋白粗萃液定量至5 mg/ml後,於接種病原菌前3天施用Ape 1蛋白200倍稀釋液,可使鳳山白菜炭疽病罹病度由72.0%降低至50.0%左右。此外,以均質機將多種植物油乳化,發現0.2% (v/v) 之乳化葵花油可顯著降低C. higginsianum PA-01的附著器形成率;進一步以相轉換法配合不同比例之非離子型界面活性劑 (Tween 80及Span 80) 製做出各種粒徑 (約150-1100 nm) 的乳化葵花油 (emulsified sunflower oil, ESO),於溫室試驗中證實粒徑約516 nm的ESO-HLB13於接種前一天施用,有最佳防治小白菜炭疽病的功效;隨施用乳化油濃度增加,防治效果亦漸增;此外,ESO-HLB13於54±2℃儲藏14天後,仍具有高穩定性與防治功效。比較上述防治資材對炭疽病菌孢子發芽的影響,發現ESO-HLB13與界面活性劑 (HLB 13) 可抑制C. higginsianum PA-01孢子發芽與產生附著器;同時也發現處理過ESO-HLB13的孢子,於發芽後仍持續延伸,沒有形成附著器。在溫室中,利用Ape 1蛋白與乳化葵花油搭配施用,發現於小白菜株齡20天時施用Ape 1蛋白200倍稀釋液及於23天時施用0.15% (v/v) ESO-HLB13,可使小白菜炭疽病的罹病度由68.0%降至36.0%左右;另外單獨於株齡23天時施用0.15% (v/v) ESO-HLB13亦可顯著降低罹病度28.0%左右。zh_TW
dc.description.tableofcontents中文摘要 i ABSTRACT iii 表次索引 viii 圖次索引 ix 第一章、前言 1 1.1. 文獻回顧 1 1.2. 實驗目的 5 第二章、材料與方法 6 2.1. 供試植物 6 2.2. 供試菌株來源及培養 6 2.3. 小白菜炭疽病接種方法及病害調查法 6 2.4. 鏈格孢菌蛋白Ape 1之製備流程與防治小白菜炭疽病之溫室試驗 7 2.4.1. 以大腸桿菌Escherichia coli ALA-01生產Ape 1蛋白之流程 7 2.4.2. 以Image J軟體評估Ape 1蛋白表現量 7 2.4.3. 蛋白表現之誘導物 (inducer) 的篩選 8 2.4.4. 在LB培養基中添加不同濃度半乳糖對Ape 1蛋白表現量的影響 8 2.4.5. Escherichia coli ALA-01在LB培養基中生產Ape 1蛋白過程的菌量與酸鹼值變化 8 2.4.6. 施用不同稀釋倍數之Ape 1蛋白防治小白菜炭疽病的效果 9 2.4.7. 不同時機施用Ape 1蛋白防治小白菜炭疽病的效果 9 2.5. 乳化植物油之製備與其防治小白菜炭疽病之溫室試驗 9 2.5.1. 試驗材料與儀器 9 2.5.2. 以均質機製備乳化植物油 10 2.5.3. 乳化植物油對Colletotrichum higginsianum PA-01孢子發芽及附著器形成的影響 11 2.5.4. 利用不同比例界面活性劑以相轉換法製作乳化葵花油 11 2.5.5. 施用不同粒徑乳化葵花油 (HLB 10-15) 防治小白菜炭疽病之效果 11 2.5.6. 不同粒徑乳化葵花油 (HLB 10-15) 的熱穩定性測試 12 2.5.7. 不同施用濃度對ESO-HLB13防治小白菜炭疽病的影響 12 2.5.8. 不同施用時機對ESO-HLB13防治小白菜炭疽病的影響 12 2.5.9. ESO-HLB13於儲藏後防治小白菜炭疽病之效果 12 2.6. 搭配不同資材防治小白菜炭疽病的效果評估 13 2.6.1. 不同資材對Colletotrichum higginsianum PA-01孢子發芽與附著器形成的影響 13 2.6.2. 溫室中施用不同資材的頻率對於小白菜炭疽病防治效果的影響 13 2.7. 統計分析 13 第三章、結果 15 3.1 鏈格孢菌蛋白Ape 1 15 3.1.1. 蛋白表現之誘導物 (inducer) 的篩選 15 3.1.2. 在LB培養基中添加不同濃度半乳糖對Ape 1蛋白表現量的影響 15 3.1.3. Escherichia coli ALA-01在LB培養基中生產Ape 1蛋白過程的菌量與酸鹼值變化 15 3.1.4. 生產Ape 1蛋白激活子的製程 16 3.1.5. 施用不同稀釋倍數之Ape 1蛋白防治小白菜炭疽病的效果 16 3.1.6. 不同時機施用Ape 1蛋白防治小白菜炭疽病的效果 16 3.2. 乳化植物油 16 3.2.1. 乳化植物油對Colletotrichum higginsianum PA-01孢子發芽及附著器形成的影響 16 3.2.2. 在不同界面活性劑比例下以相轉換法製作乳化葵花油 17 3.2.3. 施用不同粒徑乳化葵花油 (HLB 10-15) 防治小白菜炭疽病之效果 17 3.2.4. 乳化葵花油的粒徑大小與其防病效果的相關性 18 3.2.5. 不同粒徑乳化葵花油 (HLB 10-15) 的熱穩定性測試 18 3.2.6. 不同施用濃度對ESO-HLB13防治小白菜炭疽病的影響 18 3.2.7. 不同施用時機對ESO-HLB13防治小白菜炭疽病的影響 19 3.2.8. ESO-HLB13於儲藏後防治小白菜炭疽病之效果 19 3.3. 搭配不同資材防治小白菜炭疽病的效果評估 19 3.3.1. 不同資材對Colletotrichum higginsianum PA-01孢子發芽與附著器形成的影響 19 3.3.2. 溫室中不同資材搭配施用對於小白菜炭疽病防治效果的影響 19 第四章、討論 21 第五章、參考文獻 29 Appendix I. Experimental Raw Data Tables 58 Table I-1. Size distribution of five emulsified plant oils made by homogenizer 58 Table I-2. Size distribution of ESO from HLB 10 to HLB 15 (before storage) 60 Table I-3. Size distribution of ESO from HLB 10 to HLB 15 (after storage) 62 Appendix II. Experimental Raw Data Figures 64 Fig II-1. Particle distribution of five emulsified plant oils made by homogenizer. 64zh_TW
dc.language.isozh_TWzh_TW
dc.rights同意授權瀏覽/列印電子全文服務,2021-01-14起公開。zh_TW
dc.subject小白菜炭疽病zh_TW
dc.subject蛋白激活子zh_TW
dc.subject誘導抗病zh_TW
dc.subject相轉換法zh_TW
dc.subject乳化油zh_TW
dc.subjectPak-choi anthracnoseen_US
dc.subjectprotein activatoren_US
dc.subjectinduce resistanceen_US
dc.subjectphase inversion methoden_US
dc.subjectemulsified oilen_US
dc.title鏈格孢菌蛋白激活子與乳化葵花油防治小白菜炭疽病的效果zh_TW
dc.titleEfficacy of Plant Activator from Alternaria Protein and Emulsified Sunflower Oil for Controlling Pak-choi Vegetable Anthracnoseen_US
dc.typethesis and dissertationen_US
dc.date.paperformatopenaccess2019-01-14zh_TW
dc.date.openaccess2021-01-14-
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item.openairetypethesis and dissertation-
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Appears in Collections:植物醫學暨安全農業碩士學位學程
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