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標題: 細葉卷丹與台灣百合育苗之研究
The studies of plantlet for Lilium callosum and seedling cultivation for Lilium formosanum
作者: 澎山亞
Sunya Poompoung
關鍵字: 細葉卷丹;臺灣百合;增殖;栽培;保種;Lilium callosum;Lilium formosanum;Proliferation;Cultivation;Conservation
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細葉卷丹(Lilium callosum Sieb. et Zucc.)與 臺灣百合(Lilium formosanum wall.)為原生於臺灣之百合屬物種。本研究中,針對此2物種進行栽培技術之改善。調查鱗片大小對小鱗莖瓶內增殖以及小苗在溫室環境栽培下之影響。結果顯示,細葉卷丹以大鱗片(取度約9 mm)培養有最多的小鱗莖增殖數量(2.3個小鱗莖)。然而,不同大小的鱗片增殖出的鱗莖於存活率、葉片數、根數及型態上均無顯著差異。施用0.01、0.1、1 mg・L-1 之3種不同的細胞分裂素(BA、kinetin、TDZ)於大小約9 mm之鱗片,評估小鱗莖增殖效果。結果指出3種細胞分裂素使用最高濃度處理,於培養30日後均誘導癒傷組織生成。細葉卷丹鱗片培植體於0.1 mg・L-1 TDZ處理形成最多的小鱗莖(4.7個)。3種不同大小的小鱗莖於低溫層積2個月後於溫室培養4個月。結果顯示鱗莖大小介於10.0-12.0 mm 有最高的存活率(60%)、鱗莖大小以及鮮重(1.6 g)。施用1.5 g・L-1 肥料處理可獲得最大的小鱗莖直徑、葉片數、葉長以及根長。細葉卷丹馴化後以4種不同介質栽培,結果指出葉片數、小鱗莖寬度以及根數在處理間並無顯著差異。介質成分為泥炭苔(BVB):珍珠石 1:3的處理有最多的葉片數(2.8片)、小鱗莖長(18.6 mm)、寬(8.7 mm)、最長根長(6.4 cm)以及植株重(0.6 g)。綜合前述可知泥炭苔(BVB):珍珠石 1:3是栽培細葉卷丹最具增加生長量潛力的介質配方。臺灣百合(L503S和L507S)種子於5℃ 層積1個月後於混和介質中栽培,發芽率在L507S(99%)和L503S(91%)間有顯著差異。經4個月以混和介質泥炭苔(Ke):珍珠石:蛭石:碳化稻殼(2:1:1:0.5)於溫室中馴化栽培,結果顯示L507S實生苗有最高的抽莖率(50%)、葉片數(11片)及葉片長度(26 cm)。第1個花芽於栽培後17個月出現,以泥炭苔(Ke):珍珠石:蛭石:碳化稻殼(2:1:1:0.5)栽培有最多花芽形成率,L507S(41.7%)、L503S(25.0%)。因此最適合百合屬植物的栽培介質應具有良好的孔隙度與保水力。保存瀕臨絕種的百合屬植物是必需的,我們利用瓶內培養技術克服生殖障礙以及建立溫室內的栽培技術

Lilium callosum Sieb. et Zucc. and Lilium formosanum wall. are Lilium species native to Taiwan. In this study, we explored for improving the cultivation technique of these two Lilium species. We investigated the effects of bulb scale size on the bulblet proliferation in vitro and plantlet growth in greenhouse. The result showed the scale of the large size (above 9.0 mm width) formed the maximum bulblet number per scale (2.3 bulbs). However, the survival rate, leaf number and root number per scale and the morphogenesis was non significantly different in explants of different scale size. Three cytokinins (BA, kinetin and TDZ) of 0.01, 0.1 and 1 mg/l were applied on scale of above 9 mm size for evaluating the bulblet proliferation. Callus was induced at the highest concentration on the three type of cytokinins after 30 days of culture. Scale explants of Lilium callosum in the treatment of 0.1 mg/l TDZ formed the height number of bulblets (4.7 bulbs). Three different size of bulblets, after low-temperature stratification for 2 months and followed with cultivation in the greenhouse for 4 months. The results showed that the highest survival rates of 60%, bulblet size and fresh weight 1.6 g were obtained with bulblet of size 10.0-12.0 mm in diameter. The maximum diameter of bulblet, leaf number, leaf length and root length were obtained in the treatment of 1.5 g/l fertilizer input. The results of acclimatization of Lilium callosum showed non-significant difference on the number of leaves, the width of bulblet, and number of root in four kinds of mixed media. Lilium callosum on plant media contained peat moss (BVB): perlite (1:3) showed the highest of the number of leaves (2.8 leaves), bulblet size: length (18.6 mm) and width (8.7 mm), root length (6.4 cm) and whole plant weight (0.6 g). In conclusion, peat moss (BVB): perlite (1:3) had the maximum potential for growth characteristics on Lilium callosum. The result of Seedling growth on mixed media of Lilium formosanum (L500S and L507S) showed after 5℃ stratification for 1 month, there is a significant difference in germination percentages between L507S (99%) and L500S (91%). After 4 months of cultivation on mixed media for acclimatization in greenhouse, the results indicated that L507S seedlings had the highest elongation rate (50%), number of leaves (11 leaves) and leaf length (26 cm) with the composition of peat moss (Ke): perlite: vermiculite: rice husk ash (2:1:1:0.5). The first flower buds were observed after 17 months of cultivation, and the highest formation rate of flower buds was obtained in L507S (41.7%) and L500S (25.0%) on peat moss (Ke): perlite: vermiculite: rice husk ash (2:1:1:0.5). Therefore, the optimal composition of mixed media for Lilium cultivation should possess the properties of aeration porosity, water holding capacity. The conservation of endemic Lilium species is invaluable, and we applied in vitro culture to overcome the reproductive barrier and establish the cultivation technique in the greenhouse
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