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標題: Analysis of B chromosome nondisjunction induced by the r-X1 deficiency in maize
作者: Tseng, Shih-Hsuan
Peng, Shu-Fen
Cheng, Ya-Ming
關鍵字: B chromosome;Maize;Nondisjunction;Pollen FISH;r-X1 deficiency;Cell Division;Chromosomes, Plant;Zea mays
Project: Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, Volume 26, Issue 3, Page(s) 153-162.
The maize B chromosome typically undergoes nondisjunction during the second microspore division. For normal A chromosomes, the r-X1 deficiency in maize can induce nondisjunction during the second megaspore and first microspore divisions. However, it is not known whether the r-X1 deficiency also induces nondisjunction of the maize B chromosome during these cell divisions. To answer this question, chromosome numbers were determined in the progeny of r-X1/R-r female parents carrying two B chromosomes. Some of the r-X1-lacking progeny (21.2%) contained zero or two B chromosomes. However, a much higher percentage of the r-X1-containing progeny (43.4%) exhibited zero or two B chromosomes, but none displayed more than two B chromosomes. Thus, the results indicated that the r-X1 deficiency could also induce nondisjunction of the B chromosome during the second megaspore division; moreover, the B chromosome in itself could undergo nondisjunction during the same division. In addition, pollen grains from plants with two B chromosomes lacking or exhibiting the r-X1 deficiency were compared via pollen fluorescence in situ hybridization (FISH) using a B chromosome-specific probe. The results revealed that the r-X1 deficiency could induce the occurrence of B chromosome nondisjunction during the first microspore division and that the B chromosome in itself could undergo nondisjunction during the same division at a lower frequency. Our data shed more light on the behavior of the maize B chromosome during cell division.
DOI: 10.1007/s10577-017-9567-7
Appears in Collections:農藝學系

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