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標題: Production, Analysis and in Vivo Antihypertensive Evaluation of Novel Angiotensin-I-converting Enzyme Inhibitory Peptides from Porcine Brain
作者: Yu-Tse Liu
Fu-Yuan Cheng
Shiro Takeda
Hirofumi Enomoto
Gow-Chin Yen
Liang-Chuan Lin
Ryoichi Sakata
關鍵字: porcine brain;antihypertension;angiotensin-converting-enzyme;spontaneously hypertensive rats
Project: Food Science and Technology Research, 24 (3), 541-550, 2018
This study investigated the angiotensin I-converting enzyme (ACE)-inhibitory activity and antihypertensive effect of alcalase-, flavourzyme-, and protease N-digested porcine brain hydrolysates (PBHs) on spontaneously hypertensive rats (SHRs) after their oral administration. In addition, we investigated whether a peptide fraction with a molecular weight of less than 3 000 Da inhibits ACE in vitro and lowers arterial blood pressure. The results revealed that PBHs obtained after 4 h of hydrolysis with protease N (PBP4H) yielded peptides enriched with ACE-inhibitory activity (86.29 ± 5.76%). Furthermore, PBP4H had the lowest ACE-inhibitory activity IC50 value (1.945 mg/mL). Oral administration of PBP4H-100 (100 mg/kg bw/day) and low-molecular-weight peptide fractions, PBP4H < 3 000 Da (50 mg/kg bw/d) reduced the systolic blood pressure (−30 mmHg) in SHRs. Moreover, the long-term administration of PBP4H-100 and PBP4H < 3 000 Da significantly suppressed hypertension. In addition, results of the plasma ACE activity was positively related with the SBP lowering effects of PBP4Hs. Overall, these results reveal the antihypertensive effect of PBH and as a functional supplement for suppressing hypertension.
DOI: 10.3136/fstr.24.541
Appears in Collections:動物科學系

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