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標題: Kinetic analysis on precursors for iturin A production from Bacillus amyloliquefaciens BPD1
作者: Wu, Jiun-Yan
Liao, Jen-Hung
Shieh, Chwen-Jen
Hsieh, Feng-Chia
Liu, Yung-Chuan
關鍵字: Bacillus amyloliquefaciens;Iturin A;Kinetic modeling;Logistic equation;Precursor;Serine;Amino Acids;Bacillus amyloliquefaciens;Bacteriological Techniques;Bioreactors;Kinetics;Metabolic Networks and Pathways;Peptides, Cyclic
Project: Journal of bioscience and bioengineering, Volume 126, Issue 5, Page(s) 630-635.
In this study, the precursor effect for iturin A production was quantitatively analyzed. A strain identified as Bacillus amyloliquefaciens BPD1 (Ba-BPD1) was selected due to its ability to produce iturin A. The enhancement of iturin A production in a submerged culture was tested using various additives, including palmitic acid, oils, and complex amino acids. Among these, complex amino acids triggered the highest yield at 559 mg/L. The respective amino acids that contribute to the structure of iturin A were used as precursors. In fact, it was found that the addition of l-proline, l-glutamine, l-asparagine and l-serine could improve iturin A yield in the defined medium. However, during the kinetic analysis, all the amino acids exhibited a lower saturation level than l-serine, which exhibited a high saturation level at 1.2% resulting in an iturin A yield of 914 mg/L. In contrast, a negative effect was observed following the addition of l-tyrosine. To analyze the kinetic behavior of l-serine, three kinetic models were adopted: the kinetic order equation, the Langmuir kinetic equation, and a modified logistic equation. The regression results showed that the modified logistic model was the best fit for the kinetic behavior of l-serine as the major precursor, which could be further referred to the biosynthesis pathway of iturin A. Among the proposed processes for iturin A production, this study achieved the highest iturin A levels as a result of the addition of precursors.
DOI: 10.1016/j.jbiosc.2018.05.002
Appears in Collections:化學工程學系所

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