Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/99210
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dc.contributor.authorWu, Jiun-Yanzh_TW
dc.contributor.authorLiao, Jen-Hungzh_TW
dc.contributor.authorShieh, Chwen-Jenzh_TW
dc.contributor.authorHsieh, Feng-Chiazh_TW
dc.contributor.authorLiu, Yung-Chuanzh_TW
dc.contributor.author劉永銓zh_TW
dc.date2018-11-
dc.date.accessioned2019-11-06T02:33:00Z-
dc.date.available2019-11-06T02:33:00Z-
dc.identifier.urihttp://hdl.handle.net/11455/99210-
dc.description.abstractIn this study, the precursor effect for iturin A production was quantitatively analyzed. A strain identified as Bacillus amyloliquefaciens BPD1 (Ba-BPD1) was selected due to its ability to produce iturin A. The enhancement of iturin A production in a submerged culture was tested using various additives, including palmitic acid, oils, and complex amino acids. Among these, complex amino acids triggered the highest yield at 559 mg/L. The respective amino acids that contribute to the structure of iturin A were used as precursors. In fact, it was found that the addition of l-proline, l-glutamine, l-asparagine and l-serine could improve iturin A yield in the defined medium. However, during the kinetic analysis, all the amino acids exhibited a lower saturation level than l-serine, which exhibited a high saturation level at 1.2% resulting in an iturin A yield of 914 mg/L. In contrast, a negative effect was observed following the addition of l-tyrosine. To analyze the kinetic behavior of l-serine, three kinetic models were adopted: the kinetic order equation, the Langmuir kinetic equation, and a modified logistic equation. The regression results showed that the modified logistic model was the best fit for the kinetic behavior of l-serine as the major precursor, which could be further referred to the biosynthesis pathway of iturin A. Among the proposed processes for iturin A production, this study achieved the highest iturin A levels as a result of the addition of precursors.zh_TW
dc.language.isoenzh_TW
dc.relationJournal of bioscience and bioengineering, Volume 126, Issue 5, Page(s) 630-635.zh_TW
dc.subjectBacillus amyloliquefacienszh_TW
dc.subjectIturin Azh_TW
dc.subjectKinetic modelingzh_TW
dc.subjectLogistic equationzh_TW
dc.subjectPrecursorzh_TW
dc.subjectSerinezh_TW
dc.subjectAmino Acidszh_TW
dc.subjectBacillus amyloliquefacienszh_TW
dc.subjectBacteriological Techniqueszh_TW
dc.subjectBioreactorszh_TW
dc.subjectKineticszh_TW
dc.subjectMetabolic Networks and Pathwayszh_TW
dc.subjectPeptides, Cycliczh_TW
dc.titleKinetic analysis on precursors for iturin A production from Bacillus amyloliquefaciens BPD1zh_TW
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.jbiosc.2018.05.002zh_TW
dc.awards2018zh_TW
item.grantfulltextrestricted-
item.fulltextwith fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
Appears in Collections:化學工程學系所
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