Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/99212
標題: Hydroxyethyl cellulose-grafted loofa sponge-based metal affinity adsorbents for protein purification and enzyme immobilization
作者: Wei-Jie Lai
Sung-Chyr Lin
關鍵字: Loofa sponge;Immobilized metal affinity chromatography;Protein purification;Enzyme immobilization
Project: Process Biochemistry, Volume 74, November 2018, Pages 141-147
摘要: 
The development of loofa sponge-based immobilized metal affinity adsorbents was reported in this study. We showed that by grafting hydroxyethyl cellulose (HEC) to loofa sponge the chelating capacity was increased by 117% from 2520 ± 45 μmole/g to 5478 ± 77 μmole/g. Results of equilibrium adsorption study showed that the adsorption capacity of the HEC-grafted loofa sponge-based immobilized metal affinity adsorbent for the recombinant His-tagged trehalose synthase, 2.45 mg/g, was 62% higher than that of the adsorbent without HEC conjugation, 1.51 mg/g. The employment of the trehalose synthase-loaded loofa sponge-based immobilized metal affinity adsorbent as the biocatalyst for the conversion of maltose to trehalose in repeated-batch operations was conducted. It was found that after 20 cycles 70% of the initial activity was retained. The decline in activity could be attributed predominantly to the desorption of trehalose synthase from the loofa sponge-based immobilized metal affinity adsorbent.
URI: http://hdl.handle.net/11455/99212
DOI: 10.1016/j.procbio.2018.08.024
Appears in Collections:化學工程學系所

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