Please use this identifier to cite or link to this item:
標題: Hydroxyethyl cellulose-grafted loofa sponge-based metal affinity adsorbents for protein purification and enzyme immobilization
作者: Wei-Jie Lai
Sung-Chyr Lin
關鍵字: Loofa sponge;Immobilized metal affinity chromatography;Protein purification;Enzyme immobilization
Project: Process Biochemistry, Volume 74, November 2018, Pages 141-147
The development of loofa sponge-based immobilized metal affinity adsorbents was reported in this study. We showed that by grafting hydroxyethyl cellulose (HEC) to loofa sponge the chelating capacity was increased by 117% from 2520 ± 45 μmole/g to 5478 ± 77 μmole/g. Results of equilibrium adsorption study showed that the adsorption capacity of the HEC-grafted loofa sponge-based immobilized metal affinity adsorbent for the recombinant His-tagged trehalose synthase, 2.45 mg/g, was 62% higher than that of the adsorbent without HEC conjugation, 1.51 mg/g. The employment of the trehalose synthase-loaded loofa sponge-based immobilized metal affinity adsorbent as the biocatalyst for the conversion of maltose to trehalose in repeated-batch operations was conducted. It was found that after 20 cycles 70% of the initial activity was retained. The decline in activity could be attributed predominantly to the desorption of trehalose synthase from the loofa sponge-based immobilized metal affinity adsorbent.
DOI: 10.1016/j.procbio.2018.08.024
Appears in Collections:化學工程學系所

Files in This Item:
File Description SizeFormat Existing users please Login
223.pdf593.28 kBAdobe PDFThis file is only available in the university internal network   
Show full item record

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.