Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/99271
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dc.contributor.authorTang, Yi-Hsuanzh_TW
dc.contributor.authorLin, Han-Chenzh_TW
dc.contributor.authorLai, Chiao-Lingzh_TW
dc.contributor.authorChen, Po-Yuzh_TW
dc.contributor.authorLai, Chian-Huizh_TW
dc.date2018-09-30-
dc.date.accessioned2019-12-04T01:16:14Z-
dc.date.available2019-12-04T01:16:14Z-
dc.identifier.urihttp://hdl.handle.net/11455/99271-
dc.description.abstractA label-free and ultrasensitive electrochemical impedance cytosensor was developed to specifically detect the breast cancer cells MDA-MB-231 via the interaction between the mannosyl glassy carbon electrode (GCE) and the overexpressed mannose receptors on the target cell surface. The mannosyl GCE was prepared through electrografting of the amino-functionalized mannose derivatives on GCE surface in which a covalent bond was formed between carbon of the electrode and the amino group of the mannose derivative. The fluorescent microscopy indicated that the electrode is specific for MDA-MB-231 cells, with good biocompatibility for viable captured cells. The derivative with a shorter alkyl linker, mannose-C2NH2, showed a better sensitivity than that with a longer linker, mannose-C6NH2. GCE modified with amino-functionalized galactose derivative, galactose-C2NH2, shows no function to the detection of MDA-MB-231 cells. The specific interaction between the mannosyl GCE and Con A (a mannose-binding lectin) or MDA-MB-231 breast cancer cells with overexpressed mannose receptors was determined through the change of peak separation in the cyclic voltammogram or the change of charge transfer resistance in the electrochemical impedance spectra (Nyquist plot) in the electrolytes containing a reversible redox couple [Fe(CN)6]3-/[Fe(CN)6]4-. The charge transfer resistance in the Nyquist plots linearly depended on the concentration of MDA-MB-231 cells (1.0 × 10-1.0 × 105 cells mL-1, with 10 cells mL-1 being the lower detection limit). Introducing 0.1% polyethylene glycol-200 (PEG-200) was able to prevent the interference caused by 1.0 × 103 HEK-293T cells mL-1, a non-cancer cell line (control).zh_TW
dc.language.isoenzh_TW
dc.relationBiosensors & bioelectronics, Volume 116, Page(s) 100-107.zh_TW
dc.subjectCancer cell detectionzh_TW
dc.subjectCarbohydrate-cytosensorzh_TW
dc.subjectCon Azh_TW
dc.subjectLabel-freezh_TW
dc.subjectMDA-MB-231 cellzh_TW
dc.subjectMannose receptorzh_TW
dc.subjectBenzimidazoleszh_TW
dc.subjectBreast Neoplasmszh_TW
dc.subjectCarbonzh_TW
dc.subjectCell Line, Tumorzh_TW
dc.subjectConcanavalin Azh_TW
dc.subjectElectrodeszh_TW
dc.subjectFemalezh_TW
dc.subjectHEK293 Cellszh_TW
dc.subjectHumanszh_TW
dc.subjectLectins, C-Typezh_TW
dc.subjectLimit of Detectionzh_TW
dc.subjectMannosezh_TW
dc.subjectMannose-Binding Lectinszh_TW
dc.subjectOxidation-Reductionzh_TW
dc.subjectPolyethylene Glycolszh_TW
dc.subjectReceptors, Cell Surfacezh_TW
dc.subjectBiosensing Techniqueszh_TW
dc.subjectElectric Impedancezh_TW
dc.titleMannosyl electrochemical impedance cytosensor for label-free MDA-MB-231 cancer cell detectionzh_TW
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.bios.2018.05.002zh_TW
dc.awards2018zh_TW
item.grantfulltextrestricted-
item.fulltextwith fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
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