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標題: Platelet-Rich Plasma Injection in Burn Scar Areas Alleviates Neuropathic Scar Pain
作者: Huang, Shu-Hung
Wu, Sheng-Hua
Lee, Su-Shin
Lin, Yun-Nan
Chai, Chee-Yin
Lai, Chung-Sheng
Wang, Hui-Min David
關鍵字: Burn;Neuropathic Pain;PRP;Scar;Animals;Astrocytes;Burns;Chemokine CCL2;Cicatrix;Gene Expression;Humans;Neuralgia;PTEN Phosphohydrolase;Rats;Rats, Sprague-Dawley;Spinal Cord Injuries;TOR Serine-Threonine Kinases;Platelet-Rich Plasma
Project: International journal of medical sciences, Volume 15, Issue 3, Page(s) 238-247.
Objective: No effective treatments have yet been developed for burn-induced neuropathic pain. Platelet-rich plasma (PRP) has been reported to ameliorate various types of inflammation pain. However, the effect of PRP on burn-induced neuropathic pain is unclear. Methods: Burn-induced neuropathic pain Sprague-Dawley rat model was confirmed using a mechanical response test 4 weeks after the burn injuries were sustained, following which PRP was injected in the scar area. The rats were divided into four groups (n = 6) as following: Group A, Sham; Group B, Sham + PRP; Group C, Burn; and Group D, Burn + PRP. Four weeks after the PRP injection, the animals were subjected to behavior tests and then sacrificed; specimens were collected for inflammation tests, Masson's trichrome stain and chromosome 10 (PTEN) in the injured skin; and PTEN, phosphorylated mammalian target of rapamycin (p-mTOR), p38, nuclear factor κB (NFκB), chemokine (CC motif) ligand 2 (CCL2), and CCL2 cognate receptor (CCR2) in spinal cord dorsal horns through immunohistochemistry and immunofluorescence staining. Results: PRP significantly alleviated allodynia in burn-induced neuropathic pain 4 weeks after treatment, and PTEN expression in the skin and spinal cord were significantly increased in group D compared with the group C. p-PTEN, p-mTOR, and CCL2 expression in neuron cells; p-p38 and p-NFκB expression in microglia; and p-JNK and p-NFκB activation in spinal astrocytes decreased significantly in the group D compared with the group C. Conclusions: PRP is effective in treating burn-induced neuropathic pain and may be used in clinical practice.
DOI: 10.7150/ijms.22563
Appears in Collections:生醫工程研究所

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