Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/99278
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dc.contributor.authorLi, Pin-Huizh_TW
dc.contributor.authorLiu, Li-Hengzh_TW
dc.contributor.authorChang, Cheng-Chungzh_TW
dc.contributor.authorGao, Rongzh_TW
dc.contributor.authorLeung, Chung-Hangzh_TW
dc.contributor.authorMa, Dik-Lungzh_TW
dc.contributor.authorDavid Wang, Hui-Minzh_TW
dc.contributor.author王惠民zh_TW
dc.date2018-05-16-
dc.date.accessioned2019-12-04T01:42:06Z-
dc.date.available2019-12-04T01:42:06Z-
dc.identifier.urihttp://hdl.handle.net/11455/99278-
dc.description.abstractMelanogenesis is a complex physiological mechanism involving various paracrine factors. Skin cells such as keratinocytes, fibroblasts, and melanocytes communicate with one another through secreted regulators, thereby regulating the melanocytes' bio-functions. The stem cell factor (SCF) is a paracrine factor produced by fibroblasts, and its receptor, c-kit, is expressed on melanocytes. Binding of SCF to c-kit activates autophosphorylation and tyrosine kinase to switch on its signal transmission. SCF inhibition does not suppress fibroblast proliferation in MTT assay, and SCF silencing induced mRNA expressions of paracrine factor genes, HGF, NRG-1, and CRH in qPCR results. Following UVB stimulation, gene expressions of HGF, NRG, and CRH were higher than homeostasis; in particular, HGF exhibited the highest correlation with SCF variations. We detected fibroblasts regulated SCF in an autocrine-dependent manner, and the conditioned medium obtained from fibroblast culture was applied to treat melanocytes. Melanogenesis-related genes, tyrosinase and pmel17, were upregulated under conditioned mediums with SCF silencing and exposed to UVB treatments. Melanin quantities in the melanocytes had clearly increased in the pigment content assay. In conclusion, SCF silencing causes variations in both fibroblast paracrine factors and melanocyte melanogenesis, and the differences in gene expressions were observed following UVB exposure.zh_TW
dc.language.isoenzh_TW
dc.relationInternational journal of molecular sciences, Volume 19, Issue 5zh_TW
dc.subjectfibroblastszh_TW
dc.subjectmelaninzh_TW
dc.subjectmelanocyteszh_TW
dc.subjectparacrine factorszh_TW
dc.subjectstem cell factorzh_TW
dc.subjectCell Proliferationzh_TW
dc.subjectFibroblastszh_TW
dc.subjectGene Knockdown Techniqueszh_TW
dc.subjectHumanszh_TW
dc.subjectMelaninszh_TW
dc.subjectMelanocyteszh_TW
dc.subjectProto-Oncogene Proteins c-kitzh_TW
dc.subjectRNA Interferencezh_TW
dc.subjectStem Cell Factorzh_TW
dc.subjectUltraviolet Rayszh_TW
dc.subjectGene Silencingzh_TW
dc.subjectParacrine Communicationzh_TW
dc.titleSilencing Stem Cell Factor Gene in Fibroblasts to Regulate Paracrine Factor Productions and Enhance c-Kit Expression in Melanocytes on Melanogenesiszh_TW
dc.typeJournal Articlezh_TW
dc.identifier.doi10.3390/ijms19051475zh_TW
dc.awards2018zh_TW
item.grantfulltextrestricted-
item.fulltextwith fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
Appears in Collections:生醫工程研究所
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