Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/99327
標題: mRNA-Mediated Duplexes Play Dual Roles in the Regulation of Bidirectional Ribosomal Frameshifting
作者: Huang, Wan-Ping
Cho, Che-Pei
Chang, Kung-Yao
張功耀
關鍵字: Shine-Dalgarno sequence;hungry codon;mRNA-ribosome interplay;programmed ribosomal frameshifting;Base Sequence;Codon;Nucleic Acid Heteroduplexes;Open Reading Frames;Peptide Termination Factors;Protein Biosynthesis;RNA, Messenger;Ribosomes;Frameshifting, Ribosomal
Project: International journal of molecular sciences, Volume 19, Issue 12
摘要: 
In contrast to -1 programmed ribosomal frameshifting (PRF) stimulation by an RNA pseudoknot downstream of frameshifting sites, a refolding upstream RNA hairpin juxtaposing the frameshifting sites attenuates -1 PRF in human cells and stimulates +1 frameshifting in yeast. This eukaryotic functional mimicry of the internal Shine-Dalgarno (SD) sequence-mediated duplex was confirmed directly in the 70S translation system, indicating that both frameshifting regulation activities of upstream hairpin are conserved between 70S and 80S ribosomes. Unexpectedly, a downstream pseudoknot also possessed two opposing hungry codon-mediated frameshifting regulation activities: attenuation of +1 frameshifting and stimulation of a non-canonical -1 frameshifting within the +1 frameshift-prone CUUUGA frameshifting site in the absence of release factor 2 (RF2) in vitro. However, the -1 frameshifting activity of the downstream pseudoknot is not coupled with its +1 frameshifting attenuation ability. Similarly, the +1 frameshifting activity of the upstream hairpin is not required for its -1 frameshifting attenuation function Thus, each of the mRNA duplexes flanking the two ends of a ribosomal mRNA-binding channel possesses two functions in bi-directional ribosomal frameshifting regulation: frameshifting stimulation and counteracting the frameshifting activity of each other.
URI: http://hdl.handle.net/11455/99327
DOI: 10.3390/ijms19123867
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