Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/99355
DC FieldValueLanguage
dc.contributor.authorShen, Wen-Fanzh_TW
dc.contributor.authorGalula, Jedhan Ucatzh_TW
dc.contributor.authorLiu, Jyung-Hurngzh_TW
dc.contributor.authorLiao, Mei-Yingzh_TW
dc.contributor.authorHuang, Cheng-Haozh_TW
dc.contributor.authorWang, Yu-Chunzh_TW
dc.contributor.authorWu, Han-Chungzh_TW
dc.contributor.authorLiang, Jian-Jongzh_TW
dc.contributor.authorLin, Yi-Lingzh_TW
dc.contributor.authorWhitney, Matthew Tzh_TW
dc.contributor.authorChang, Gwong-Jen Jzh_TW
dc.contributor.authorChen, Sheng-Renzh_TW
dc.contributor.authorWu, Shang-Rungzh_TW
dc.contributor.author趙黛瑜zh_TW
dc.contributor.authorChao, Day-Yuzh_TW
dc.date2018-
dc.date.accessioned2020-02-04T03:21:34Z-
dc.date.available2020-02-04T03:21:34Z-
dc.identifier.urihttp://hdl.handle.net/11455/99355-
dc.description.abstractDengue fever is caused by four different serotypes of dengue virus (DENV) which is the leading cause of worldwide arboviral diseases in humans. Virus-like particles (VLPs) containing flavivirus prM/E proteins have been demonstrated to be a potential vaccine candidate; however, the structure of dengue VLP is poorly understood. Herein VLP derived from DENV serotype-2 were engineered becoming highly matured (mD2VLP) and showed variable size distribution with diameter of ~31 nm forming the major population under cryo-electron microscopy examination. Furthermore, mD2VLP particles of 31 nm diameter possess a T = 1 icosahedral symmetry with a groove located within the E-protein dimers near the 2-fold vertices that exposed highly overlapping, cryptic neutralizing epitopes. Mice vaccinated with mD2VLP generated higher cross-reactive (CR) neutralization antibodies (NtAbs) and were fully protected against all 4 serotypes of DENV. Our results highlight the potential of 'epitope-resurfaced' mature-form D2VLPs in inducing quaternary structure-recognizing broad CR NtAbs to guide future dengue vaccine design.zh_TW
dc.language.isoenzh_TW
dc.relationeLife, Volume 7zh_TW
dc.subjectbroad antibody responsezh_TW
dc.subjectcryoEMzh_TW
dc.subjectdenguezh_TW
dc.subjectinfectious diseasezh_TW
dc.subjectmicrobiologyzh_TW
dc.subjectmolecular biophysicszh_TW
dc.subjectmousezh_TW
dc.subjectstructural biologyzh_TW
dc.subjectvaccinezh_TW
dc.subjectviruszh_TW
dc.subjectvirus-like particlezh_TW
dc.subjectAmino Acid Sequencezh_TW
dc.subjectAnimalszh_TW
dc.subjectAntibodies, Monoclonalzh_TW
dc.subjectAntibodies, Neutralizingzh_TW
dc.subjectDengue Vaccineszh_TW
dc.subjectDengue Viruszh_TW
dc.subjectEpitopeszh_TW
dc.subjectFemalezh_TW
dc.subjectImmunizationzh_TW
dc.subjectMice, Inbred BALB Czh_TW
dc.subjectSerotypingzh_TW
dc.subjectSolventszh_TW
dc.subjectSurvival Analysiszh_TW
dc.subjectVaccines, Virus-Like Particlezh_TW
dc.subjectViral Envelope Proteinszh_TW
dc.subjectVirionzh_TW
dc.titleEpitope resurfacing on dengue virus-like particle vaccine preparation to induce broad neutralizing antibodyzh_TW
dc.typeJournal Articlezh_TW
dc.identifier.doi10.7554/eLife.38970zh_TW
dc.awards2018zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextwith fulltext-
item.languageiso639-1en-
item.grantfulltextrestricted-
Appears in Collections:微生物暨公共衛生學研究所
Files in This Item:
File Description SizeFormat Existing users please Login
368.pdf1.42 MBAdobe PDFThis file is only available in the university internal network    Request a copy
Show simple item record
 

Google ScholarTM

Check

Altmetric

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.