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標題: Genotype I of Japanese Encephalitis Virus Virus-like Particles Elicit Sterilizing Immunity against Genotype I and III Viral Challenge in Swine
作者: Fan, Yi-Chin
Chen, Jo-Mei
Lin, Jen-Wei
Chen, Yi-Ying
Wu, Guan-Hong
Su, Kuan-Hsuan
Chiou, Ming-Tang
Wu, Shang-Rung
Yin, Ji-Hang
Liao, Jiunn-Wang
Chang, Gwong-Jen J
Chiou, Shyan-Song
關鍵字: Animals;Antibodies, Neutralizing;CHO Cells;COS Cells;Chlorocebus aethiops;Cricetinae;Cricetulus;Disease Models, Animal;Encephalitis Virus, Japanese;Encephalitis, Japanese;Female;Genotype;Japanese Encephalitis Vaccines;Mice;Mice, Inbred BALB C;RNA, Viral;Swine;Vaccination;Vero Cells;Virion;Cross Protection
Project: Scientific reports, Volume 8, Issue 1, Page(s) 7481.
Swine are a critical amplifying host involved in human Japanese encephalitis (JE) outbreaks. Cross-genotypic immunogenicity and sterile protection are important for the current genotype III (GIII) virus-derived vaccines in swine, especially now that emerging genotype I (GI) JE virus (JEV) has replaced GIII virus as the dominant strain. Herein, we aimed to develop a system to generate GI JEV virus-like particles (VLPs) and evaluate the immunogenicity and protection of the GI vaccine candidate in mice and specific pathogen-free swine. A CHO-heparan sulfate-deficient (CHO-HS(-)) cell clone, named 51-10 clone, stably expressing GI-JEV VLP was selected and continually secreted GI VLPs without signs of cell fusion. 51-10 VLPs formed a homogeneously empty-particle morphology and exhibited similar antigenic activity as GI virus. GI VLP-immunized mice showed balanced cross-neutralizing antibody titers against GI to GIV viruses (50% focus-reduction micro-neutralization assay titers 71 to 240) as well as potent protection against GI or GIII virus infection. GI VLP-immunized swine challenged with GI or GIII viruses showed no fever, viremia, or viral RNA in tonsils, lymph nodes, and brains as compared with phosphate buffered saline-immunized swine. We thus conclude GI VLPs can provide sterile protection against GI and GIII viruses in swine.
DOI: 10.1038/s41598-018-25596-1
Appears in Collections:微生物暨公共衛生學研究所

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